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Obnovit | RAW

Biochemical Instrumentation and Robotics

Lecture Excercise/laboratory
2019, Summer Semester
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Credits 3
Hours per week 0 / 2 / 0
Examination MC
Study Language Czech
Level []
Guarantor doc. Mgr. Ing. Štěpánka Kučková, Ph.D.

Summary

The course is focused on solving practical problems in the biochemical laboratory. The course summarizes the findings of analytical chemistry, biochemistry and basic robotic systems.

Syllabus

1. Basic terms (measurements, quantities, characteristics of analytical methods), preparation of distilled water, optical methods
2. Biosensors, potentiometric sensors, the principles of pH measurements and maintenance of electrodes
3. Optical, thermal and piezoelectric transducers, sensors based on sensitive polymer layer, requirements for ideal biosensor
4. Procedures used for sample preparation for analysis, filtration, ultrafiltration, dialysis, sonication, microwave devices
5. Robotics, automation, system miniaturization
6. Separation methods, flow cytometry, centrifuges and centrifugal methods, rotors, field flow fractionation
7. Electrophoresis I: basic terms, separation modes
8. Electrophoresis II: detection, blotting, processing and storage of electropherograms
9. Chromatography I: basic terms, methods, TLC, different types of chromatographs
10. Chromatography II: liquid chromatography, Van Deemter equation, diagnostic of problems with chromatographic separation, gel chromatography
11. Chromatography III: Ion exchange chromatography, sorbents for hydrophobic chromatography, chromatofocusing, chelation ion chromatography
12. Detectors used in chromatography, derivatization of analytes, maintenance rules, selection of appropriate equipment
13. Multidimensional separation techniques in proteomics, combining of different separation techniques and detectors
14. Preparative methods, methods of instruments adjusting, troubleshooting

Literature

R: Kodíček M., Karpenko V., Biofyzikální chemie, Academia, 2000, 80-200-0791-1
R: Káš J., Kodíček M., Valentová O., Laboratorní techniky biochemie, Vysoká škola chemicko-technologická v Praze, 2006, 80-7080-586-2
A: Churáček J. a kol., Analytická separace látek, SNTL, Praha 1990, 80-03-00569-8

VŠCHT Praha
Technická 5
166 28 Praha 6 – Dejvice
IČO: 60461373
DIČ: CZ60461373

Datová schránka: sp4j9ch

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