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Obnovit | RAW

Immunochemistry: Laboratory

Lecture Excercise/laboratory
2019, Summer Semester
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Credits 3
Hours per week 0 / 0 / 4
Examination MC
Study Language Czech
Level []
Guarantor doc. Ing. Igor Hochel, CSc.

Summary

The course is focused on i) acquiring of knowledge and skills in the discipline, ii) detailed description of antigen, hapten and all significant isotypes of antibodies, iii) utilization of antibodies for isolation and preparation of pure preparations, iv) utilization of antibodies in food and clinical testing and environmental monitoring, v) mastering the procedures of various immunochemical assays.

Syllabus

1. Immunoprecipitation in liquid phase
2. Double diffusion according to Ouchterlony
3. Radial immunodiffusion according to podle Mancini
4. Electrosyneresis
5. Immunoelectrodiffusion according to Laurell
6. Indirect competitive ELISA
7. Non-competitive ELISA
8. GLISA Rapid test
9. SDS-PAGE of proteins, blotting and immunochemical detection of analytes
10. Immunoagglutination

Literature

R: Daussant J., Desvaux F.-X., Introduction to immunochemical techniques for medical diagnosis, food quality control and environmental testing, 2007, ICT Press, 9788070806418

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DIČ: CZ60461373

Datová schránka: sp4j9ch

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