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Obnovit | RAW

Plant Biochemistry

Lecture Excercise/laboratory
2019, Summer Semester
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Credits 3
Hours per week 2 / 0 / 0
Examination Ex
Study Language Czech
Level []
Guarantor prof. Ing. Tomáš Macek, CSc.

Summary

The subject is aiming to understand the of basic differences between the metabolism of plants and other organisms, especialy in the field of energy exploitation, production abilities and response to stress. The goal is to prepare the students for effective exploitation of specific advantages of plants, ranging from food industry to environmental protection, including the use or detection of genetically modified plants. Photosynthesis is the key process for life on Earth, for that reason it is given a special block of lectures.

Syllabus

1. Anatomy, cytology and physiology of plants (cell wall, membranes, organelles), uptake of nutrients, transport within the whole plant
2. Preparation and exploitation of explant cultures, callus, suspension, protoplasts, genetic studies, signal transduction, metabolite production, biotransformation, environmental studies, application in breeding and multiplication
3. Energy conversion, photosynthesis, respiration and photorespiration
4. Carbon metabolism (biosynthesis of sucrose, cellulose, starch, lignins, lipids etc.)
5. Metabolism of nitrogen, sulphur in plants
6. Main groups of hormones (auxins, cytokinins, ethylene, abscisic acid, gibberellins, brassinosteroids etc.), formation and degradation, effect of hormones and growth regulators
7. Receptors and signal transduction mediators (G-proteins, phospholipid pathway), protein kinases, signalling pathways of plant hormones
8. Biosynthesis of some important natural products in plants, importance of the so called secondary metabolites for plants and for relationships within the environment, effects on plants and Man
9. The genome of nucleus, plastids and mitochondria, organisation, expression, proteosynthesis and protein modification, targeting of protein transport
10. Gene transfer methods in plants, preparation of transgenic plants
11. Genetically modified plants, importance, risks, benefits and advantages
12. Abiotic stress (water deficit, increased salt concentration, cold, heat, oxidative stress), biotic stress, defence mechanisms in plants
13. Metabolisation of organic compounds, degradation, activation of promutagens, heavy metal accumulation, phytoremediation, rhizoremediation. Specific aspects of plant defence and protection, pesticides, herbicides
14. Senescence, programmed cell death, post-harvest changes, chlorophyl degradation

Literature

R: Buchanan B.B., Gruissem W., Jones R.L., Biochemistry & molecular biology of plants, American Society of Plant Physiologists, Rockville, 2015
R: Voet D.,Voet J.G., Biochemistry, John Wiley & Sons, New York, 2004
R: Kodíček M., Valentová O., Hynek R. Biochemie: chemický pohled na biologický svět. VŠCHT Praha 2015
R: Macková M., Dowling D., Macek T., Phytoremediation and Rhizoremediation, Theoretical Background, Springer, Berlin, 2006
A: Macek T., Kotrba P., Svatos A., Novakova M., Demnerova K., and Mackova M., Novel roles for genetically modified plants in environmental protection. Trends in Biotechnology 26, 146-152, (2008)
A: Vodrážka Z., Biochemie, Academia, 1996, 80-200-0600-1
A: Macek, T., Macková, M., Káš, J.: Exploitation of plants for the removal of organics in environmental remediation. Biotechnol. Advances (2000), 18 (1), 23-35
A: Kodíček M. Biochemické pojmy, výkladový slovník, VŠCHT Praha

VŠCHT Praha
Technická 5
166 28 Praha 6 – Dejvice
IČO: 60461373
DIČ: CZ60461373

Datová schránka: sp4j9ch

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