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Obnovit | RAW

Advanced Biochemistry: Laboratory

Lecture Excercise/laboratory
2020, Summer Semester
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Credits 3
Hours per week 0 / 0 / 5
Examination MC
Study Language Czech
Level []
Guarantor doc. Ing. Jan Lipov, Ph.D.
doc. Dr. Ing. Zuzana Novotná
Supplemental electronic materials available at UCT Prague's e-learning

Summary

Tha aim of the subject is to expand the basic knowledge of the biochemical course and to adopt new techniques of modern biochemistry and to amend the spectrum of techniques of biochemistry and molecular biology used in research laboratories.

Syllabus

1. Good laboratory practice, safety
2. Theoretical introduction to the laboratory tasks
3. Chromatography of biomacromolecules, evaluation of purification steps
4. Electrophoretic analysis of erythrocyte membrane proteins
5. Basic characterization of purified preparates of proteins
6. Enzyme kinetics (allosteric enzymes)
7. Modern methods of protein identification
8. Physical & chemical properties of proteins
9. Immunotechniques
10.Basic techniques of molecular biology
11.Isolation of subcellular particles
12.Study of basic metabolic events
13.Methods of determination of catalytical activity of enzymes
14.Evaluation, test and free discussion

Literature

R: Káš J., Kodíček M., Valentová O.: Laboratorní techniky biochemie. 1. vyd. Vysoká škola chemicko-technologická v Praze, Praha 2006. ISBN 80-7080-586-2

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