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Obnovit | RAW

Seminar I

Lecture Excercise/laboratory
2019, Summer Semester
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Credits 2
Hours per week 0 / 2 / 0
Examination MC
Study Language Czech
Level []
Guarantor prof. Ing. Pavel Kotrba, Ph.D.
Ing. Tereza Leonhardt, Ph.D.
Ing. Jiří Šantrůček, Ph.D.

Summary

The course objectives include: introduction to fundamental bioinformatic approaches and tools allowing analyzing nucleotide and protein sequences, including critical interpretation of analysis outputs; survey of potential uses of bioinformatic analyses to address experimental tasks; and detailed insight into practical aspects of related molecular biology and proteomic methods and approaches.

Syllabus

1. Bioinformatics, cross-database search platforms for data mining.
2. Databases and software for nucleotide sequence analyses.
3. Gene function predictions, prediction of promoters and regulatory elements.
4. Design and practical aspects of PCR experiment.
5. Central dogma upturned: a strategy to isolate coding sequence.
6. Practical aspects and method selection in DNA and DNA-binding protein analyses.
7. Practical aspects and method selection in RNA and transcript level analyses.
8. Proteomic servers and databases. Tools for protein sequence alignments.
9. Protein modifications. In silico prediction and experimental proof.
10. Protein secondary structure and hydropathy profile. In silico prediction and experimental proof.
11. Protein quaternary structure and oligomerization state. In silico prediction and experimental proof.
12. Membrane proteins. Determination of protein localization in biological membrane, the surface properties of proteins and detergents.
13. Native state of protein. Intracellular localization and interactions of protein.
14. Laboratory work with proteins. Protein storage and sample preparation for analyses.

Literature

R: Cvrčková F., Úvod do praktické bioinformatiky, Academia, 2006, 8020013601
A: Campbel A.M., Heyer L.J., Discovering genomics, proteomics and bioinformatics, CSHL Press, 0805347224
A: Wilkins M., et al., ed., Proteome Research: New Frontiers in Functional Genomics , Springer-Verlag, 1997, 3540627537

VŠCHT Praha
Technická 5
166 28 Praha 6 – Dejvice
IČO: 60461373
DIČ: CZ60461373

Datová schránka: sp4j9ch

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