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Obnovit | RAW

Genetic Engineering

Lecture Excercise/laboratory
2019, Summer Semester
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Credits 3
Hours per week 2 / 0 / 0
Examination Ex
Study Language Czech
Level []
Guarantor prof. Ing. Tomáš Ruml, CSc.

Summary

The subject is focused on understanding of principles of manipulation with nucleic acids and their analysis. It covers information about various methods of introducing genes into diverse cell types, determination of gene expression and analysis. The goal is to deliver information about genetic engineering techniques in order to facilitate the choce of the optimal method for a particular application. The lectures cover basic methods of isolation, analysis and modification of nucleic acids and special applications as gene modification for detection or affinity purification of gene product, study of interaction of proteins and nucleic acids, or gene therapies.

Syllabus

1. Recapitulation of genetic principles.
2. Bacterial plasmid vectors.
3. Basic operations with DNA.
4. Labeling of nucleic acids and application of probes.
5. Analysis of DNA sequences.
6. Principles of work with RNA.
7. Polymerase chain reaction and its applications.
8. Expression in microbial cells, markers, fusion proteins.
9. Tissue cultures and their use for gene expression.
10. Principle of construction of transgenic organisms.
11. Mutations, directed mutagenesis.
12. Genome mapping, restriction analysis.
13. Product detection - metabolic labeling, electrophoretic and immunochemical methods.
14. Methods for study in protein and nucleic acids interactions.

Literature

R: Ruml T., Rumlová M., Pačes V.: Genové inženýrství; VŠCHT Praha 2002
R: Green M. R., Sambrook J., Molecular Cloning: A Laboratory Manual (Fourth Edition); Cold Spring Harbor Laboratory Press 2012

VŠCHT Praha
Technická 5
166 28 Praha 6 – Dejvice
IČO: 60461373
DIČ: CZ60461373

Datová schránka: sp4j9ch

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