Cannabinoids (THC, CBD, HHC,...)

The determination of cannabinoids is mostly performed in cannabis and cannabis products, food and beverages, confectionery, dietary supplements (oil-based or dried herbs), extracts, concentrates and resins, vapes, collectibles, but also in blood plasma and forensic samples.

Currently, we can target and quantify up to 74 cannabinoids. Among these compounds you can also find substances regulated by Government (CZ) Regulation No. 463/2013 Coll., and No. 11/2025 Coll., such as THC, HHC, MDMB-PINACA, and others. Furthermore, Commission Regulation (EU) 2023/915 sets maximum limits for the sum of delta-9-tetrahydrocannabinol (Δ9-THC) and delta-9-tetrahydrocannabinolic acid (Δ9-THCA), expressed as THC equivalents, in cannabis products.

In addition, we can also measure the total profile of phytocannabinoids and other bioactive compounds in the samples; qualitative screening by UHPLC-HRMS/MS (KM 21i) of cannabinoids and other bioactive compounds.

For the quantitative determination of cannabinoids by UHPLC-HRMS(MS) (KM21) and UHPLC-DAD (KM 29), we currently offer several ranges of determinations:

Scope 1: basic range I

•  4 major natural cannabinoids by the UHPLC-HRMS method (KM21)

CBD (cannabidiol); CBDA (cannabidiolic acid); CBD equivalents; trans-Δ9-THC (trans-delta-9-tetrahydrocannabinol); Δ9-THCA-A (delta-9-tetrahydrocannabinolic acid-A); THC equivalents

Scope 2: basic range II

•  natural cannabinoids by the UHPLC-HRMS method (KM21)

CBD (cannabidiol); CBDA (cannabidiolic acid); CBD equivalents; Δ9-THC (suma cis- a trans-delta-9-tetrahydrocannabinol); Δ9-THCA-A (delta-9-tetrahydrocannabinolic acid-A); CBN (cannabinol); CBNA (cannabinolic acid)

Scope 3: compounds from the group of tetrahydrocannabinols

•  9 cannabinols by the UHPLC-DAD method (KM29)

trans -Δ9-THC (trans -delta-9-tetrahydrocannabinol); cis-Δ9-THC (cis-delta-9-tetrahydrocannabinol); exo-THC (exo-tetrahydrocannabinol); (6aR,9R)-Δ10-THC ((6aR,9R)-delta-10-tetrahydrocannabinol); (6aR,9S)-Δ10-THC ((6aR,9S)-delta-10-tetrahydrocannabinol); Δ8-THC (delta-8-tetrahydrocannabinol); 10-OH-∆8-THC (10α-hydroxy-delta-8-tetrahydrocannabinol); Δ9-THCA-A (delta-9-tetrahydrocannabinolic acid-A); THC equivalents

Scope 4: spectrum of 10 phytocannabinoids in concentrates / isolates from medical cannabis

10 phytocannabinoids Ph.Eur. 11 by the UHPLC-DAD method (KM29)

CBD (cannabidiol); CBDA (cannabidiolic acid); CBD equivalents; trans-Δ9-THC (trans-delta-9-tetrahydrocannabinol); Δ9-THCA-A (delta-9-tetrahydrocannabinolic acid-A); THC equivalents; CBN (cannabinol); CBNA (cannabinolic acid); CBG (cannabigerol); CBGA (cannabigerolic acid); CBDV (cannabidivarine); CBC (cannabichromene).

Scope 5: extended basic range

• 18 cannabinoids by the UHPLC-HRMS method (KM21):

CBD (cannabidiol); CBDA (cannabidiolic acid); CBD equivalents; trans-Δ9-THC (trans-delta-9-tetrahydrocannabinol); Δ9-THCA-A (delta-9-tetrahydrocannabinolic acid-A); THC equivalents; cis-Δ9-THC (cis-delta-9-tetrahydrocannabinol); Δ8-THC (delta-8-tetrahydrocannabinol); Δ9-THCV (delta-9-tetrahydrocannabivarine); THCVA (tetrahydrocannabivarinic acid); CBN (cannabinol); CBNA (cannabinolic acid); CBG (cannabigerol); CBGA (cannabigerolic acid); CBDV (cannabidivarine); CBDVA (cannabidivarinic acid); CBC (cannabichromene); CBCA (cannabichromenic acid); CBL (cannabicyclol); CBLA (cannabicyclolic acid).

Scope 6: major cannabinoids (49) 

• 49 cannabinoids by the UHPLC-HRMS method (KM21)

CBD (cannabidiol); CBDA (cannabidiolic acid); CBD equivalents; trans-Δ9-THC (trans-delta-9-tetrahydrocannabinol); cis-Δ9-THC (cis-delta-9-tetrahydrocannabinol); exo-THC (exo-tetrahydrocannabinol); (6aR,9R)-Δ10-THC ((6aR,9R)-delta-10-tetrahydrocannabinol); (6aR,9S)-Δ10-THC ((6aR,9S)-delta-10-tetrahydrocannabinol); Δ9-THCA-A (delta-9-tetrahydrocannabinolic acid-A); Δ8-THCA-A (delta-8-tetrahydrocannabinolic acid-A); THC equivalents; Δ8-THC (delta-8-tetrahydrocannabinol); Δ9-THCV (delta-9-tetrahydrocannabivarine); Δ8-THCV (delta-8-tetrahydrocannabivarine); THCVA (tetrahydrocannabivarinic acid); CBN (cannabinol); CBNA (cannabinolic acid); CBG (cannabigerol); CBGA (cannabigerolic acid); CBDV (cannabidivarine); CBDVA (cannabidivarinic acid); CBC (cannabichromene); CBCA (cannabichromenic acid); CBL (cannabicyclol); CBLA (cannabicyclolic acid); CBCV (cannabichromevarine); CBCVA (cannabichromevarinic acid); CBGAQ (cannabigerol quinone acid); CBV (cannabivarine); CBVA (cannabivarinic acid); CBGO (cannabigerorcine); CBGOA (cannabigerorcinic acid); CBGV (cannabigerovarine); CBGVA (cannabigerovarinic acid); CBCO (cannabichromeorcin); CBCOA (cannabichromeorcinic acid); CBT (cannabicitran); CBE (cannabielsoin); CBGB (cannabigerobutol); CBDB (cannabidibutol); Δ9-THCB (delta-9-tetrahydrocannabutol); CBDH (cannabidihexol); Δ9-THCH (delta-9-tetrahydrocannabihexol); CBND (cannabinodiol); CBGM (cannabigerol monomethyl ether); CBNM (cannabinol monomethyl ether); CBDP (cannabidiphorol); Δ9-THCP (delta-9-tetrahydrocannabiphorol); Δ8-THCP (delta-8-tetrahydrocannabiphorol); (R)-HHC (9(R)-hexahydrokanabinol); (S)-HHC (9(S)-hexahydrokanabinol).

Scope 7: (semi)synthetic cannabinoids

•  30 cannabinoids by the  UHPLC-HRMS method (KM21)

exo-THC (exo-tetrahydrocannabinol); (R)-HHC (9(R)-hexahydrokanabinol); (S)-HHC (9(S)-hexahydrokanabinol); (R)-HHCH (9(R)- hexahydrocannabihexol); (S)-HHCH (9(S)- hexahydrocannabihexol); (R)-HHCP (9(R)-hexahydrocannabiphorol); (S)-HHCP (9(S)-hexahydrocannabiphorol); (R)-HHCPO (9(R)-hexahydrocannabiphorol acetate); (S)-HHCPO (9(S)-hexahydrocannabiphorol acetate); (R)-HHCO (9(R)-hexahydrocannabinol acetate); (S)-HHCO (9(S)-hexahydrocannabinol acetate); (R)-H4CBD (1(R)-tetrahydrocannabidiol); (S)-H4CBD (1(S)-tetrahydrocannabidiol); 9-OH-HHC (9-alfa-hydroxy hexahydrocannabinol); Δ9-THCP (delta-9-tetrahydrocannabiphorol); Δ8-THCP (delta-8-tetrahydrocannabiphorol); Δ9-THC-O (delta-9-tetrahydrocannabinol acetát); Δ9-THCH (delta-9-tetrahydrocannabihexol); Δ9-THC-C8  (delta-9-tetrahydrocannabinol-C8); Δ8-THC-C8  (delta-8-tetrahydrocannabinol-C8); Δ9-THCB (delta-9-tetrahydrocannabutol); (R)-HHCB (9(R)-hexahydrocannabutol); (S)-HHCB (9(S)-hexahydrocannabutol); (R)-HHC-C8 (9(R)-hexahydrocannabinol-C8); (S)-HHC-C8 (9(S)-hexahydrocannabinol-C8); 10(R)-OH-9(S)-HHC  (10(R)hydroxy9(S)hexahydrocannabinol); 10(S)-OH-9(R)-HHC  (10(S)hydroxy9(R)hexahydrocannabinol); 10-OH-∆8-THC (10α-hydroxy-delta-8-tetrahydrocannabinol); MDMB-PINACA, MDMB-4en-PINACA.

Scope 8: all cannabinoids (74)

• 74 cannabinoids by the  UHPLC-HRMS method (KM21)

CBD (cannabidiol); CBDA (cannabidiolic acid); CBD equivalents; trans-Δ9-THC (trans-delta-9-tetrahydrocannabinol); cis-Δ9-THC (cis-delta-9-tetrahydrocannabinol); exo-THC (exo-tetrahydrocannabinol); (6aR,9R)-Δ10-THC ((6aR,9R)-delta-10-tetrahydrocannabinol); (6aR,9S)-Δ10-THC ((6aR,9S)-delta-10-tetrahydrocannabinol); Δ9-THCA-A (delta-9-tetrahydrocannabinolic acid-A); Δ8-THCA-A (delta-8-tetrahydrocannabinolic acid-A); THC equivalents; Δ8-THC (delta-8-tetrahydrocannabinol); Δ9-THCV (delta-9-tetrahydrocannabivarine); Δ8-THCV (delta-8-tetrahydrocannabivarine); THCVA (tetrahydrocannabivarinic acid); CBN (cannabinol); CBNA (cannabinolic acid); CBG (cannabigerol); CBGA (cannabigerolic acid); CBDV (cannabidivarine); CBDVA (cannabidivarinic acid); CBC (cannabichromene); CBCA (cannabichromenic acid); CBL (cannabicyclol); CBLA (cannabicyclolic acid); CBCV (cannabichromevarine); CBCVA (cannabichromevarinic acid); CBGAQ (cannabigerol quinone acid); CBV (cannabivarine); CBVA (cannabivarinic acid); CBGO (cannabigerorcine); CBGOA (cannabigerorcinic acid); CBGV (cannabigerovarine); CBGVA (cannabigerovarinic acid); CBCO (cannabichromeorcin); CBCOA (cannabichromeorcinic acid); CBT (cannabicitran); CBE (cannabielsoin); CBGB (cannabigerobutol); CBDB (cannabidibutol); Δ9-THCB (delta-9-tetrahydrocannabutol); CBDH (cannabidihexol); Δ9-THCH (delta-9-tetrahydrocannabihexol); CBND (cannabinodiol); CBGM (cannabigerol monomethyl ether); CBNM (cannabinol monomethyl ether); CBDP (cannabidiphorol); Δ9-THCP (delta-9-tetrahydrocannabiphorol); Δ8-THCP (delta-8-tetrahydrocannabiphorol); (R)-HHC (9(R)-hexahydrokanabinol); (S)-HHC (9(S)-hexahydrokanabinol); (R)-HHCH (9(R)- hexahydrocannabihexol); (S)-HHCH (9(S)- hexahydrocannabihexol); (R)-HHCP (9(R)-hexahydrocannabiphorol); (S)-HHCP (9(S)-hexahydrocannabiphorol); (R)-HHCPO (9(R)-hexahydrocannabiphorol acetate); (S)-HHCPO (9(S)-hexahydrocannabiphorol acetate); (R)-HHCO (9(R)-hexahydrocannabinol acetate); (S)-HHCO (9(S)-hexahydrocannabinol acetate); (R)-H4CBD (1(R)-tetrahydrocannabidiol); (S)-H4CBD (1(S)-tetrahydrocannabidiol); 9-OH-HHC (9-alfa-hydroxy hexahydrocannabinol); Δ9-THC-O (delta-9-tetrahydrocannabinol acetát); Δ9-THCH (delta-9-tetrahydrocannabihexol); Δ9-THC-C8  (delta-9-tetrahydrocannabinol-C8); Δ8-THC-C8  (delta-8-tetrahydrocannabinol-C8); Δ9-THCB (delta-9-tetrahydrocannabutol); (R)-HHCB (9(R)-hexahydrocannabutol); (S)-HHCB (9(S)-hexahydrocannabutol); (R)-HHC-C8 (9(R)-hexahydrocannabinol-C8); (S)-HHC-C8 (9(S)-hexahydrocannabinol-C8); 10(R)-OH-9(S)-HHC  (10(R)hydroxy9(S)hexahydrocannabinol); 10(S)-OH-9(R)-HHC  (10(S)hydroxy9(R)hexahydrocannabinol); 10-OH-∆8-THC (10α-hydroxy-delta-8-tetrahydrocannabinol); MDMB-PINACA, MDMB-4en-PINACA.